作者：林竑翊, 李明智, 郭慧华, 袁军, 汪世华, 王秀娜
单位：福建农林大学生命科学学院 福建省病原真菌与真菌毒素重点实验室，福建 福州 350002
关键词：Aspergillus flavus,锌指蛋白,黄曲霉毒素,生长发育,碳源,酸碱
基金：福建省自然科学基金(2024J01397),福建省自然科学基金(2022J01594),国家自然科学基金(31800040),福建农林大学科技创新专项基金(KFB23081),福建农林大学科技创新专项基金(KFB22048XA)
C2H2型转录因子AZF1在真菌中具有调控生长发育、次级代谢及致病等多种生物学功能，但其在黄曲霉中的作用尚不明确。同源序列比对发现，AFLA_054800编码的蛋白与里氏木霉中的AZF1蛋白具有50%的同源性，将其命名为AflAZF1。本研究发现，AflAZF1基因缺失菌株(ΔAflAZF1)的菌落大小与野生型无明显差异，但分生孢子产量显著下降，且无法形成菌核。同时，ΔAflAZF1菌株的黄曲霉毒素AFB1产量明显升高。在以葡萄糖、阿拉伯糖、甘露糖、蔗糖和木糖为碳源时，ΔAflAZF1菌株的产孢能力相较于野生型显著下降，但在乳糖和甘油中显著上升。此外，在pH 5和pH 11条件下，ΔAflAZF1菌株的分生孢子数量显著少于野生型。研究结果表明，AflAZF1能够调控分生孢子和菌核的形成及AFB1的生物合成，还通过影响产孢能力调节对不同碳源的利用和对极端pH条件的适应性。这一发现揭示了AflAZF1在黄曲霉生长发育与毒素合成中的关键作用，为防治黄曲霉污染及制定新型生物防控策略提供了理论依据。"/>template{display:none;}.mag-rich-xref-fn { cursor: pointer;}//长视频$(document).ready(function(){if($("#showLongArticleVideo") && $("#showLongArticleVideo").length && $("#showLongArticleVideo").length>0){mag_ajax_update({ele_id:'showLongArticleVideo',url:mag_currentQikanUrl() + "/CN/article/showLongArticleVideo.do?id="+$("#articleId").val()});}});//可视化图谱$(document).ready(function(){if($("#showArticle_knowledgeMap") && $("#showArticle_knowledgeMap").length && $("#showArticle_knowledgeMap").length>0){jQuery.getJSON("/Jwxb/CN/lexeme/existsCnctstInArticle.do?articleID="+$("#articleId").val(), null, function(json){if(json.result){$("#showArticle_knowledgeMap").show();}});}});$(document).ready(function(){var metaDataStr = $.trim($("#article_metaJson").val());if(metaDataStr!=''){eval("window.metaData="+metaDataStr);}if(window.metaData && (!window.metaData.authorNotesCommon_cn && !window.metaData.authorNotesCorresp_cn && window.metaData.authorNotes_cn)){window.metaData.authorNotesCommon_cn = window.metaData.authorNotes_cn;}if(window.metaData && (!window.metaData.authorNotesCommon_en && !window.metaData.authorNotesCorresp_en && window.metaData.authorNotes_en)){window.metaData.authorNotesCommon_en = window.metaData.authorNotes_en;}var _nlmdtdXml = $("#article_nlmdtdXml").val();var magDir = _nlmdtdXml.replace(/\/[^\/]+$/,'');window.metaData.magDir = magDir;new mag_vue({ el: '#metaVue', //dataUrl: mag_currentQikanUrl() + '/EN/article/getRichHtmlJson.do?articleId='+$("#articleId").val(), data:window.metaData, loading:true, doTextFun:function(text){ return doDataJsonText(text); 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来源：2026年第2期
《菌物学报》期刊编辑部
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