作者：邱松林, 胡世成, 童彩玲, 焦迎春
单位：1 厦门海洋职业技术学院海洋生物学院 海洋资源保护与生态治理福建省高等学校应用技术工程中心 厦门市智慧渔业重点实验室，福建 厦门 361100,2 青海大学农牧学院，青海 西宁 810016
关键词：胞内多糖,大肥菇,体外低氧模型,条件优化,酵解特征
基金：国家自然科学基金地区项目(31960471)
大肥菇胞内多糖(IPS)具有较优的抗缺氧活性，但由于分子量过大不能被人体直接吸收，为进一步从小分子层面去研究大肥菇胞内多糖的抗缺氧机理，作者以缺氧条件下喂养的雄性小鼠和雌性小鼠的肠道菌群构建体外低氧酵解模型，优化了条件，并解析了多糖在不同性别小鼠肠道菌群中的消化特征。雄性小鼠组(M组)酵解37 h、初始多糖含量24.0 mg/mL、菌群和培养基比6:4和菌群浓度30%，雌性小鼠组(F组)酵解时间39 h、初始多糖含量26.0 mg/mL、菌群浓度和培养基比例8:5和菌群浓度30%为最优体外酵解条件。在酵解终点处，M组和F组总糖及还原糖消耗率均高于90%，胞内多糖降解产生了葡萄糖、葡萄糖醛酸、甘露糖、半乳糖、半乳糖醛酸、果糖、核糖、木糖和阿拉伯糖9种单糖及醛酸(MSUA)，M组含量水平依次主要由葡萄糖醛酸≥木糖>果糖组成，F组含量水平依次主要由甘露糖>果糖>半乳糖醛酸组成，且F组的单糖及醛酸总量高出M组近1倍；同时还生成6种短链脂肪酸(SCFAs)，分别是乙酸、丙酸、异丁酸、丁酸、异戊酸和戊酸，M组和F组主要由乙酸、丁酸、丙酸及异戊酸组成，与M组相比，F组的乙酸含量水平远高于其他3种，而与F组相比，M组中丁酸含量水平较高；M组和F组低氧肠道菌群的组成相似但各自的相对占比不同，多糖促进了M组中双歧杆菌属、普雷沃氏菌属相对丰度增加，及F组中普雷沃氏菌属及罗斯氏菌属相对丰度显著增加，降低了M组和F组中大肠杆菌-志贺氏杆菌属相对丰度。相较于雄性小鼠的低氧肠道菌群，雌性小鼠的低氧肠道菌群在体外酵解模型中具有不同的酵解特性，但都可以为胞内多糖的体外模拟消化研究提供理论基础。"/>template{display:none;}.mag-rich-xref-fn { cursor: pointer;}//长视频$(document).ready(function(){if($("#showLongArticleVideo") && $("#showLongArticleVideo").length && $("#showLongArticleVideo").length>0){mag_ajax_update({ele_id:'showLongArticleVideo',url:mag_currentQikanUrl() + "/CN/article/showLongArticleVideo.do?id="+$("#articleId").val()});}});//可视化图谱$(document).ready(function(){if($("#showArticle_knowledgeMap") && $("#showArticle_knowledgeMap").length && $("#showArticle_knowledgeMap").length>0){jQuery.getJSON("/Jwxb/CN/lexeme/existsCnctstInArticle.do?articleID="+$("#articleId").val(), null, function(json){if(json.result){$("#showArticle_knowledgeMap").show();}});}});$(document).ready(function(){var metaDataStr = $.trim($("#article_metaJson").val());if(metaDataStr!=''){eval("window.metaData="+metaDataStr);}if(window.metaData && (!window.metaData.authorNotesCommon_cn && !window.metaData.authorNotesCorresp_cn && window.metaData.authorNotes_cn)){window.metaData.authorNotesCommon_cn = window.metaData.authorNotes_cn;}if(window.metaData && (!window.metaData.authorNotesCommon_en && !window.metaData.authorNotesCorresp_en && window.metaData.authorNotes_en)){window.metaData.authorNotesCommon_en = window.metaData.authorNotes_en;}var _nlmdtdXml = $("#article_nlmdtdXml").val();var magDir = _nlmdtdXml.replace(/\/[^\/]+$/,'');window.metaData.magDir = magDir;new mag_vue({ el: '#metaVue', //dataUrl: mag_currentQikanUrl() + '/EN/article/getRichHtmlJson.do?articleId='+$("#articleId").val(), data:window.metaData, loading:true, doTextFun:function(text){ return doDataJsonText(text); 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来源：2025年第5期
《菌物学报》期刊编辑部
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