作者：王成凤, 刘夏, 张曦予, 董杰, 张实润, 杨晓敏, 吕为, 刘佳艳, 李宗菊
单位：1 云南大学生命科学学院，云南 昆明 650500,2 云南大学国际河流与生态安全研究院，云南 昆明 650500,3 云南大学生态与环境学院，云南 昆明 650500
关键词：皱皮粗柄白蚁伞,蚁巢,关联物质,关联通路,复合氨基酸,糖类,促生作用
基金：国家自然科学基金(31560575),云南省自然科学基金(2014FA020)
白蚁伞为云南名贵野生菌，与白蚁互惠共生于白蚁巢中，其子实体的发育至今仍然是一个难解之谜。为了解白蚁伞子实体与蚁巢的物质流，本文应用液相质谱(LC-MS)技术，对皱皮粗柄白蚁伞Termitomyces robustus子实体及其生长基质蚁巢的代谢物谱进行了分析。子实体(JZ)和蚁巢(YC)中共检测到2 023个代谢物，其中JZ特有物质(JZS) 284个，YC特有物质(YCS) 317个，二者共有显著差异物质(GDS) 322个。以相对含量≥0.5的化合物为例，利用Cytoscape (v3.10.0)软件分别展示了43个JZS及YCS中39个物质、29个GDS中19个物质间的显著相关性。KEGG富集到18条共有通路中的40个JZS、YCS及GDS物质，通过氨基酸生物合成及代谢(ABM)、次级代谢产物的生物合成(BSM)及ABC转运3大类通路发生关联。18条通路中，12条与ABM相关，2条与ABC转运中糖代谢相关。选取参与ABM通路的9种氨基酸(苯丙氨酸、异亮氨酸、酪氨酸、脯氨酸、苏氨酸、缬氨酸、色氨酸、亮氨酸、谷氨酸)、参与ABC转运的3种糖(蔗糖、山梨醇、海藻糖)及蚁巢水提液对纯培养菌丝体的促生作用研究表明，每升基础培养基中加入0.2-0.6 g 9种复合氨基酸，20 g葡萄糖+5 g蔗糖或山梨醇或海藻糖、50-150 mL蚁巢水提液，对菌丝体的生长都有显著的促进作用；但添加150 mL蚁巢水提液对菌丝的促生效果显著优于复合氨基酸及糖处理组(0.6 g复合氨基酸+20 g葡萄糖+5 g海藻糖)及复合氨基酸处理组(0.6 g/L复合氨基酸)。284个JZS、317个YCS及322个GDS，特别是以上98个物质在子实体及蚁巢中显著关联，推测其对白蚁伞子实体的发育有重要的调控作用；复合氨基酸及山梨醇或海藻糖对纯培养菌丝体促生效果显著，但次于蚁巢水提液。以上研究为今后进一步分析与白蚁伞子实体生长发育相关的信号分子，探明白蚁伞与白蚁的共生机理，规模化人工培养白蚁伞等提供了理论依据。"/>template{display:none;}.mag-rich-xref-fn { cursor: pointer;}//长视频$(document).ready(function(){if($("#showLongArticleVideo") && $("#showLongArticleVideo").length && $("#showLongArticleVideo").length>0){mag_ajax_update({ele_id:'showLongArticleVideo',url:mag_currentQikanUrl() + "/CN/article/showLongArticleVideo.do?id="+$("#articleId").val()});}});//可视化图谱$(document).ready(function(){if($("#showArticle_knowledgeMap") && $("#showArticle_knowledgeMap").length && $("#showArticle_knowledgeMap").length>0){jQuery.getJSON("/Jwxb/CN/lexeme/existsCnctstInArticle.do?articleID="+$("#articleId").val(), null, function(json){if(json.result){$("#showArticle_knowledgeMap").show();}});}});$(document).ready(function(){var metaDataStr = $.trim($("#article_metaJson").val());if(metaDataStr!=''){eval("window.metaData="+metaDataStr);}if(window.metaData && (!window.metaData.authorNotesCommon_cn && !window.metaData.authorNotesCorresp_cn && window.metaData.authorNotes_cn)){window.metaData.authorNotesCommon_cn = window.metaData.authorNotes_cn;}if(window.metaData && (!window.metaData.authorNotesCommon_en && !window.metaData.authorNotesCorresp_en && window.metaData.authorNotes_en)){window.metaData.authorNotesCommon_en = window.metaData.authorNotes_en;}var _nlmdtdXml = $("#article_nlmdtdXml").val();var magDir = _nlmdtdXml.replace(/\/[^\/]+$/,'');window.metaData.magDir = magDir;new mag_vue({ el: '#metaVue', //dataUrl: mag_currentQikanUrl() + '/EN/article/getRichHtmlJson.do?articleId='+$("#articleId").val(), data:window.metaData, loading:true, doTextFun:function(text){ return doDataJsonText(text); 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来源：2025年第3期
《菌物学报》期刊编辑部
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