作者：张蓓宁, 孔庆辉, 王志佳, 刘丰慧, 刘锁珠, 谭占坤, 商振达
单位：<sup class=\mag-xml-aff-label-sup added-by-sys\>1</sup> 西藏农牧学院动物科学学院， 西藏 林芝 860000,<sup class=\mag-xml-aff-label-sup added-by-sys\>2</sup> 西藏高原饲料加工工程研究中心， 西藏 林芝 860000
关键词：雅江雪牛,库氏毕赤酵母,产酶条件优化,全基因组测序,纤维降解能力
基金：西藏农牧学院研究生创新项目(YJS2023-19),西藏农牧学院学科建设项目(YJSXK2025-17)
为扩大饲料资源，有效利用秸秆资源，本研究以西藏雅江雪牛为研究对象，利用羧甲基纤维素钠选择性培养基从雅江雪牛瘤胃中筛选纤维素降解菌，并探究菌株的产酶特性及其对秸秆纤维素的降解能力。研究结果得到，从雅江雪牛瘤胃中共筛选出6株可以崩解滤纸条的菌株，其中一株(XZ6)被鉴定为库氏毕赤酵母Pichia kudriavzevii。单因素产酶条件优化确定了该菌株的最优产木聚糖酶条件，即在温度为33 ℃、培养时间为18 h、摇床转速为240 r/min、接种量为1% (体积比)时，菌株产木聚糖酶的活性最高为11.55 U/mL，比优化前提高了12倍；产β-葡聚糖酶的活性最高为1.82 U/mL，比优化前提高了7倍；产β-甘露聚糖酶活性最高为2.07 U/mL，比优化前提高了3倍。全基因组测序得到该菌株共注释到408个糖苷水解酶基因和352个糖基转移酶基因。利用菌株XZ6的粗酶液发酵青稞秸秆与玉米秸秆，可以显著降低两种秸秆中粗纤维、中性洗涤纤维和酸性洗涤纤维的含量。综合来看，菌株XZ6具有降解秸秆纤维的能力。"/>template{display:none;}.mag-rich-xref-fn { cursor: pointer;}//长视频$(document).ready(function(){if($("#showLongArticleVideo") && $("#showLongArticleVideo").length && $("#showLongArticleVideo").length>0){mag_ajax_update({ele_id:'showLongArticleVideo',url:mag_currentQikanUrl() + "/CN/article/showLongArticleVideo.do?id="+$("#articleId").val()});}});//可视化图谱$(document).ready(function(){if($("#showArticle_knowledgeMap") && $("#showArticle_knowledgeMap").length && $("#showArticle_knowledgeMap").length>0){jQuery.getJSON("/Jwxb/CN/lexeme/existsCnctstInArticle.do?articleID="+$("#articleId").val(), null, function(json){if(json.result){$("#showArticle_knowledgeMap").show();}});}});$(document).ready(function(){var metaDataStr = $.trim($("#article_metaJson").val());if(metaDataStr!=''){eval("window.metaData="+metaDataStr);}if(window.metaData && (!window.metaData.authorNotesCommon_cn && !window.metaData.authorNotesCorresp_cn && window.metaData.authorNotes_cn)){window.metaData.authorNotesCommon_cn = window.metaData.authorNotes_cn;}if(window.metaData && (!window.metaData.authorNotesCommon_en && !window.metaData.authorNotesCorresp_en && window.metaData.authorNotes_en)){window.metaData.authorNotesCommon_en = window.metaData.authorNotes_en;}var _nlmdtdXml = $("#article_nlmdtdXml").val();var magDir = _nlmdtdXml.replace(/\/[^\/]+$/,'');window.metaData.magDir = magDir;new mag_vue({ el: '#metaVue', //dataUrl: mag_currentQikanUrl() + '/EN/article/getRichHtmlJson.do?articleId='+$("#articleId").val(), data:window.metaData, loading:true, doTextFun:function(text){ return doDataJsonText(text); 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来源：2025年第11期
《菌物学报》期刊编辑部
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