菌物学报杂志2025年第11期:基于MNP分子标记的茯苓种质遗传多样性分析与菌株鉴定-菌物学报杂志社

菌物学报杂志2025年第11期:基于MNP分子标记的茯苓种质遗传多样性分析与菌株鉴定

发布日期:

作者：王淼, 汪琦, 董彩虹

单位：1 中国科学院微生物研究所微生物多样性与资源创新利用全国重点实验室，北京 100101,2 中国科学院大学，北京 100049,3 湖北省中医院，湖北武汉 430071

关键词：茯苓,种质,遗传相似度,菌株鉴定,多核苷酸多态性

基金：湖北省中医药科研项目(ZY2025Q030),湖北省重点研发计划(2024BBB087)

茯苓Wolfiporia hoelen是我国传统的药食同源真菌，具有重要的药用和经济价值。尽管我国已实现茯苓的大规模栽培，但仍存在品种混杂、遗传背景不清及优良菌株缺乏等问题。因此，开展茯苓遗传多样性研究，建立高效、准确的菌株鉴定方法具有重要意义。本研究选取中国主要栽培菌株、野生菌株以及日本的茯苓菌株为实验材料，基于单核苷酸多态性(SNP)进行了茯苓遗传多样性分析，并首次构建了茯苓多核苷酸多态性(MNP)分子标记库。群体遗传结构分析表明，供试茯苓群体可分为2个主要遗传群体，其中群体A呈现典型的克隆遗传特征。MNP分子标记库包括654个高特异性MNP位点，菌株间遗传相似性(GS)在9.6%-100%之间，其中群体A内菌株间的GS高达99.8%-100%。继代培养和原生质体再生菌株的基因组重测序分析证实，短期无性繁殖不影响菌株MNP基因型的稳定性。本研究建立的MNP分子鉴定体系为茯苓的品种权保护和菌株鉴别提供了可靠的技术支撑，并为茯苓杂交育种亲本的选择及种质创新奠定了基础。"/>template{display:none;}.mag-rich-xref-fn { cursor: pointer;}//长视频$(document).ready(function(){if($("#showLongArticleVideo") && $("#showLongArticleVideo").length && $("#showLongArticleVideo").length>0){mag_ajax_update({ele_id:'showLongArticleVideo',url:mag_currentQikanUrl() + "/CN/article/showLongArticleVideo.do?id="+$("#articleId").val()});}});//可视化图谱$(document).ready(function(){if($("#showArticle_knowledgeMap") && $("#showArticle_knowledgeMap").length && $("#showArticle_knowledgeMap").length>0){jQuery.getJSON("/Jwxb/CN/lexeme/existsCnctstInArticle.do?articleID="+$("#articleId").val(), null, function(json){if(json.result){$("#showArticle_knowledgeMap").show();}});}});$(document).ready(function(){var metaDataStr = $.trim($("#article_metaJson").val());if(metaDataStr!=''){eval("window.metaData="+metaDataStr);}if(window.metaData && (!window.metaData.authorNotesCommon_cn && !window.metaData.authorNotesCorresp_cn && window.metaData.authorNotes_cn)){window.metaData.authorNotesCommon_cn = window.metaData.authorNotes_cn;}if(window.metaData && (!window.metaData.authorNotesCommon_en && !window.metaData.authorNotesCorresp_en && window.metaData.authorNotes_en)){window.metaData.authorNotesCommon_en = window.metaData.authorNotes_en;}var _nlmdtdXml = $("#article_nlmdtdXml").val();var magDir = _nlmdtdXml.replace(/\/[^\/]+$/,'');window.metaData.magDir = magDir;new mag_vue({ el: '#metaVue', //dataUrl: mag_currentQikanUrl() + '/EN/article/getRichHtmlJson.do?articleId='+$("#articleId").val(), data:window.metaData, loading:true, doTextFun:function(text){ return doDataJsonText(text); 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菌物学报杂志2025年第11期:基于MNP分子标记的茯苓种质遗传多样性分析与菌株鉴定

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