作者：梁倩倩, 丁玲强, 田丽雯, 王玉臻, 牛鑫, 单华佳
单位：1 河西学院甘肃省应用真菌工程实验室，甘肃 张掖 734000,2 河西学院甘肃省食用菌遗传育种重点实验室，甘肃 张掖 734000,3 河西学院生命科学与工程学院，甘肃 张掖 734000,4 河西学院祁连山菌物保护利用协同创新基地，甘肃 张掖 734000,5 河西学院农业与生态工程学院，甘肃 张掖 734000
关键词：形态学鉴定,ITS鉴定,生物学特性,驯化栽培
基金：甘肃省科技厅重点研发计划(23YFNG0006),张掖市重大技术攻关揭榜挂帅制项目(ZY2022JBGS07),甘肃省省级重点人才项目(2023RCXM34),甘肃省自然科学基金(23JRRG0031),甘肃省教育厅高校教师创新基金(2024A151)
本研究对采自祁连山的一株野生菌株进行形态学和分子生物学鉴定，通过单因素试验探讨不同因素对菌丝生长的影响，通过正交试验优化菌丝最优生长配方，进行驯化栽培并对接种量和栽培料进行优化。结果表明：野生菌LQQ2019129鉴定为双孢蘑菇Agaricus bisporus，子实体为褐色；在试验范围内，菌丝生长的最适温度为28 ℃，最适碳源为蔗糖，最适氮源为酵母粉，最适无机盐为硫酸钙，最适硫酸钙浓度为1.6 g/L；菌丝生长的最优培养基配方为马铃薯200 g/L，蔗糖15 g/L，酵母粉4 g/L，硫酸钙1.6 g/L，水1 000 mL，pH自然；子实体在麦草和玉米秸秆上均可出菇，最适接菌量为麦草5%，玉米秸秆10%，麦草较玉米秸秆更适于该菌株的栽培。"/>template{display:none;}.mag-rich-xref-fn { cursor: pointer;}//长视频$(document).ready(function(){if($("#showLongArticleVideo") && $("#showLongArticleVideo").length && $("#showLongArticleVideo").length>0){mag_ajax_update({ele_id:'showLongArticleVideo',url:mag_currentQikanUrl() + "/CN/article/showLongArticleVideo.do?id="+$("#articleId").val()});}});//可视化图谱$(document).ready(function(){if($("#showArticle_knowledgeMap") && $("#showArticle_knowledgeMap").length && $("#showArticle_knowledgeMap").length>0){jQuery.getJSON("/Jwxb/CN/lexeme/existsCnctstInArticle.do?articleID="+$("#articleId").val(), null, function(json){if(json.result){$("#showArticle_knowledgeMap").show();}});}});$(document).ready(function(){var metaDataStr = $.trim($("#article_metaJson").val());if(metaDataStr!=''){eval("window.metaData="+metaDataStr);}if(window.metaData && (!window.metaData.authorNotesCommon_cn && !window.metaData.authorNotesCorresp_cn && window.metaData.authorNotes_cn)){window.metaData.authorNotesCommon_cn = window.metaData.authorNotes_cn;}if(window.metaData && (!window.metaData.authorNotesCommon_en && !window.metaData.authorNotesCorresp_en && window.metaData.authorNotes_en)){window.metaData.authorNotesCommon_en = window.metaData.authorNotes_en;}var _nlmdtdXml = $("#article_nlmdtdXml").val();var magDir = _nlmdtdXml.replace(/\/[^\/]+$/,'');window.metaData.magDir = magDir;new mag_vue({ el: '#metaVue', //dataUrl: mag_currentQikanUrl() + '/EN/article/getRichHtmlJson.do?articleId='+$("#articleId").val(), data:window.metaData, loading:true, doTextFun:function(text){ return doDataJsonText(text); 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来源：2025年第1期
《菌物学报》期刊编辑部
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