作者：张思瑶, 余盛武, 魏玉莲
单位：1 中国科学院沈阳应用生态研究所 中国科学院森林生态与管理重点实验室 辽宁 沈阳 110016,2 中国科学院大学 北京 100049,3 钱江源-百山祖国家公园龙泉保护中心 浙江 龙泉 323700
关键词：药用真菌,锈革孔菌科,代谢产物,成分分析
基金：国家自然科学基金(32270017),辽宁省应用基础研究计划(2022JH2/101300182)
桑黄是一类著名的药用真菌，多糖、黄酮、三萜是桑黄主要的活性成分，三者的药用价值受到了广泛关注。东亚木层孔菌Phellinus orientoasiaticus是生长于蔷薇科活立木上的大型真菌，其外观形态与桑黄高度形似，但其药用价值的研究鲜有报道。本研究以东北地区研究广泛的鲍姆桑黄Sanghuangporus baumii和忍冬桑黄S. lonicericola野生子实体为对比研究材料，以粗多糖、总黄酮、总三萜的含量及其抗氧化活性为对比研究指标，探究东亚木层孔菌的药用价值。鲍姆桑黄粗多糖含量为4.64 mg/g、忍冬桑黄为2.94 mg/g、东亚木层孔菌为3.68 mg/g；鲍姆桑黄的总黄酮含量为65.53 mg/g、忍冬桑黄为187.74 mg/g、东亚木层孔菌为46.19 mg/g；鲍姆桑黄总三萜含量为5.38 mg/g、忍冬桑黄为15.87 mg/g、东亚木层孔菌为10.17 mg/g。通过HPLC分析3种子实体黄酮成分，发现鲍姆桑黄总黄酮中可能含有芹菜素和槲皮素这2种成分；忍冬桑黄总黄酮中可能含有芹菜素、柚皮素这2种成分；东亚木层孔菌总黄酮中可能含有芹菜素和芦丁这2种成分。比较3种真菌子实体黄酮和多糖的DPPH自由基清除能力、ABTS自由基清除能力、超氧阴离子清除能力和羟自由基清除能力等抗氧化活性，结果发现忍冬桑黄粗多糖和东亚木层孔菌粗多糖抗氧化活性较好，鲍姆桑黄总黄酮抗氧化活性较好，忍冬桑黄和东亚木层孔菌总三萜抗氧化活性较好。本研究显示，东亚木层孔菌的粗多糖、总黄酮和总三萜含量均比较高，抗氧化活性较好，具有良好的药用应用前景。"/>template{display:none;}.mag-rich-xref-fn { cursor: pointer;}//长视频$(document).ready(function(){if($("#showLongArticleVideo") && $("#showLongArticleVideo").length && $("#showLongArticleVideo").length>0){mag_ajax_update({ele_id:'showLongArticleVideo',url:mag_currentQikanUrl() + "/CN/article/showLongArticleVideo.do?id="+$("#articleId").val()});}});//可视化图谱$(document).ready(function(){if($("#showArticle_knowledgeMap") && $("#showArticle_knowledgeMap").length && $("#showArticle_knowledgeMap").length>0){jQuery.getJSON("/Jwxb/CN/lexeme/existsCnctstInArticle.do?articleID="+$("#articleId").val(), null, function(json){if(json.result){$("#showArticle_knowledgeMap").show();}});}});$(document).ready(function(){var metaDataStr = $.trim($("#article_metaJson").val());if(metaDataStr!=''){eval("window.metaData="+metaDataStr);}if(window.metaData && (!window.metaData.authorNotesCommon_cn && !window.metaData.authorNotesCorresp_cn && window.metaData.authorNotes_cn)){window.metaData.authorNotesCommon_cn = window.metaData.authorNotes_cn;}if(window.metaData && (!window.metaData.authorNotesCommon_en && !window.metaData.authorNotesCorresp_en && window.metaData.authorNotes_en)){window.metaData.authorNotesCommon_en = window.metaData.authorNotes_en;}var _nlmdtdXml = $("#article_nlmdtdXml").val();var magDir = _nlmdtdXml.replace(/\/[^\/]+$/,'');window.metaData.magDir = magDir;new mag_vue({ el: '#metaVue', //dataUrl: mag_currentQikanUrl() + '/EN/article/getRichHtmlJson.do?articleId='+$("#articleId").val(), data:window.metaData, loading:true, doTextFun:function(text){ return doDataJsonText(text); 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来源：2024年第2期
《菌物学报》期刊编辑部
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