作者：颜丽涵,赵博荣,杨虹,邓丹丹,杨飞,杨官娥
单位：山西医科大学药学院，山西 晋中 030600
关键词：内生真菌,制备条件,原生质体紫外诱变,鼠李素-3-O-鼠李糖苷,原生质体转化
基金：中央引导地方科技发展项目(YDZX),中央引导地方科技发展项目(20201400001443),山西省基础研究计划项目(202103021223241),山西省国际科技合作项目(201803D421065)
鼠李素-3-O-鼠李糖苷(alpha-rhamnetin-3-O-rhamnoside, ARR)是从北桑寄生Loranthus tanakae中分离得到的黄酮类化合物，有良好的抗炎药理活性，具有较大的研究价值。从北桑寄生中分离出的内生菌能够产生与北桑寄生相同或相似的化学成分，其中有6种内生菌具备产鼠李素-3-O-鼠李糖苷的能力。由于微生物发酵具有高效、绿色可持续的优点，北桑寄生内生菌可以作为北桑寄生植物的替代资源。本研究以一株北桑寄生青霉属内生真菌ZS108为研究对象，利用酶解法制备其原生质体，探究了原生质体制备的适宜条件，采用聚乙二醇(PEG)转化法对不同酶解时间的原生质体进行初步转化，经DNA提取及PCR验证表明质粒成功整合到内生真菌ZS108基因组中。然后对原生质体进行紫外诱变，经过筛选最终得到14株鼠李素-3-O-鼠李糖苷产量比出发菌株高的突变菌株，正突变率为16.47%。其中突变菌株UV39产量最高，达到3.93 mg/L，比出发菌株高6.02倍。最后采用薄层色谱法和LC-MC法对突变菌株产鼠李素-3-O-鼠李糖苷的能力进行了验证。通过原生质体紫外诱变的方法选育高产鼠李素-3-O-鼠李糖苷菌株，为微生物发酵法生产鼠李素-3-O-鼠李糖苷早日实现工业化生产打下了坚实的基础。"/>template{display:none;}.mag-rich-xref-fn { cursor: pointer;}//长视频$(document).ready(function(){if($("#showLongArticleVideo") && $("#showLongArticleVideo").length && $("#showLongArticleVideo").length>0){mag_ajax_update({ele_id:'showLongArticleVideo',url:mag_currentQikanUrl() + "/CN/article/showLongArticleVideo.do?id="+$("#articleId").val()});}});//可视化图谱$(document).ready(function(){if($("#showArticle_knowledgeMap") && $("#showArticle_knowledgeMap").length && $("#showArticle_knowledgeMap").length>0){jQuery.getJSON("/Jwxb/CN/lexeme/existsCnctstInArticle.do?articleID="+$("#articleId").val(), null, function(json){if(json.result){$("#showArticle_knowledgeMap").show();}});}});$(document).ready(function(){var metaDataStr = $.trim($("#article_metaJson").val());if(metaDataStr!=''){eval("window.metaData="+metaDataStr);}if(window.metaData && (!window.metaData.authorNotesCommon_cn && !window.metaData.authorNotesCorresp_cn && window.metaData.authorNotes_cn)){window.metaData.authorNotesCommon_cn = window.metaData.authorNotes_cn;}if(window.metaData && (!window.metaData.authorNotesCommon_en && !window.metaData.authorNotesCorresp_en && window.metaData.authorNotes_en)){window.metaData.authorNotesCommon_en = window.metaData.authorNotes_en;}var _nlmdtdXml = $("#article_nlmdtdXml").val();var magDir = _nlmdtdXml.replace(/\/[^\/]+$/,'');window.metaData.magDir = magDir;new mag_vue({ el: '#metaVue', //dataUrl: mag_currentQikanUrl() + '/EN/article/getRichHtmlJson.do?articleId='+$("#articleId").val(), data:window.metaData, loading:true, doTextFun:function(text){ return doDataJsonText(text); 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来源：2024年第1期
《菌物学报》期刊编辑部
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