作者：贾景辉,刘志强,王雨曦,魏玉莲,袁海生
单位：1 辽宁大学生命科学院，辽宁 沈阳 110036,2 中国科学院沈阳应用生态研究所 中国科学院森林生态与管理重点实验室，辽宁 沈阳 110164
关键词：槐生拜尔孔菌,化学成分,甾体类,结构鉴定,酪氨酸酶抑制活性
基金：中国博士后科学基金面上项目(2022M713296),沈阳市科技计划项目(22-322-3-07),辽宁省应用基础研究计划项目(2022JH2/101300182)
本试验研究了槐生拜尔孔菌(槐耳) Vanderbylia robiniophila大米固体发酵物中的甾体类化合物及其酪氨酸酶抑制活性。采用硅胶柱层析、ODS柱层析、凝胶柱层析和高效液相色谱(high performance liquid chromatography, HPLC)等色谱法分离纯化槐生拜尔孔菌发酵产物，利用质谱(mass spectrometer, MS)、核磁共振(nuclear magnetic resonance, NMR)等方法进行结构鉴定得到10个甾体类化合物。化合物的结构分别鉴定为：isocyathisterol (1)、fortisterol (2)、7α-methoxy-5α,6α- epoxyergosta-8(14),22-diene-3β-triol (3)、(3β,5α,6β,22E)-6-methoxyergosta-7,22-diene-3,5-diol (4)、citreoanthrasteroid B (5)、(22E,24S)-5α,8α-epidioxy-24-methyl-cholesta-6,9(11),22-trien-3β-ol (6)、(22E,24R)-5α,8α-epidioxy-ergosta-6,22-dien-3β-ol (7)、5α,6α-epoxy-3β-hydroxy-(22E, 24R)-ergosta-8,22- dien-7-one (8)、calvasterol A (9)和phomopsterone A (10)，均是属内首次分离获得。对化合物1-10进行酪氨酸酶抑制活性的测定，其中化合物1抑制酪氨酸酶的IC50值为(25.46±3.86) μmol/L，化合物3抑制酪氨酸酶的IC50值为(31.58±4.65) μmol/L，其活性优于阳性药熊果苷[IC50值为(58.17±6.09) μmol/L]，可作为黑色素瘤新药制剂开发的候选化合物。"/>template{display:none;}.mag-rich-xref-fn { cursor: pointer;}//长视频$(document).ready(function(){if($("#showLongArticleVideo") && $("#showLongArticleVideo").length && $("#showLongArticleVideo").length>0){mag_ajax_update({ele_id:'showLongArticleVideo',url:mag_currentQikanUrl() + "/CN/article/showLongArticleVideo.do?id="+$("#articleId").val()});}});//可视化图谱$(document).ready(function(){if($("#showArticle_knowledgeMap") && $("#showArticle_knowledgeMap").length && $("#showArticle_knowledgeMap").length>0){jQuery.getJSON("/Jwxb/CN/lexeme/existsCnctstInArticle.do?articleID="+$("#articleId").val(), null, function(json){if(json.result){$("#showArticle_knowledgeMap").show();}});}});$(document).ready(function(){var metaDataStr = $.trim($("#article_metaJson").val());if(metaDataStr!=''){eval("window.metaData="+metaDataStr);}if(window.metaData && (!window.metaData.authorNotesCommon_cn && !window.metaData.authorNotesCorresp_cn && window.metaData.authorNotes_cn)){window.metaData.authorNotesCommon_cn = window.metaData.authorNotes_cn;}if(window.metaData && (!window.metaData.authorNotesCommon_en && !window.metaData.authorNotesCorresp_en && window.metaData.authorNotes_en)){window.metaData.authorNotesCommon_en = window.metaData.authorNotes_en;}var _nlmdtdXml = $("#article_nlmdtdXml").val();var magDir = _nlmdtdXml.replace(/\/[^\/]+$/,'');window.metaData.magDir = magDir;new mag_vue({ el: '#metaVue', //dataUrl: mag_currentQikanUrl() + '/EN/article/getRichHtmlJson.do?articleId='+$("#articleId").val(), data:window.metaData, loading:true, doTextFun:function(text){ return doDataJsonText(text); 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来源：2023年第9期
《菌物学报》期刊编辑部
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