作者：陈浩然,李忻洋,刘珍,刘世红,刘可欣,董盈,王玉龙
单位：安徽农业大学微生物防治省重点实验室，安徽 合肥 230036
关键词：蛹虫草,单孢菌株,交配型,继代培养
基金：国家自然科学基金(32102274),安徽省教育厅自然科学科研项目(KJ2020A0129),安徽省教育厅自然科学科研项目(YJS20210237),安徽农业大学大学生创新训练计划(202110364240)
蛹虫草菌株在继代培养过程中极易退化。本研究选取5株蛹虫草菌株作为出发菌株，基于单孢分离和显微技术从每株出发菌株中获得50株单孢菌株。通过PCR方法对单孢菌株进行交配型类型鉴定，全部为单一交配型单孢菌株，且不同出发菌株分离得到的MAT1-1和MAT1-2交配型单孢菌株比例差异较大，分别为27:23、34:16、42:8、28:22和7:43。从不同出发菌株获得的单孢菌株中随机选择MAT1-1和MAT1-2交配型单孢菌株各5株(共计50株)，进行菌落直径、产孢量和虫草素含量测定。与出发菌株相比较，14株单孢菌株菌落直径具有显著差异(其中10株显著减小)，24株产孢量具有显著差异且全部下降，29株单孢菌株虫草素含量具有显著差异(其中21株显著下降)。进一步，将50株单孢菌株连续继代培养，测定菌株菌落直径、产孢量和虫草素含量，计算第七代与第一代比值评估菌株性状稳定性。结果表明，与出发菌株相比较，14株单孢菌株菌落直径比值具有显著差异且全部增加，41株单孢菌株产孢量比值具有显著差异(其中40株显著下降)，44株虫草素含量比值具有显著差异且全部下降。本研究表明同一菌株中的不同单孢菌株个体之间，在生长、产孢和次级代谢产物含量等方面存在差异，且单孢菌株的继代培养性状稳定性比出发菌株差。"/>template{display:none;}.mag-rich-xref-fn { cursor: pointer;}//长视频$(document).ready(function(){if($("#showLongArticleVideo") && $("#showLongArticleVideo").length && $("#showLongArticleVideo").length>0){mag_ajax_update({ele_id:'showLongArticleVideo',url:mag_currentQikanUrl() + "/CN/article/showLongArticleVideo.do?id="+$("#articleId").val()});}});//可视化图谱$(document).ready(function(){if($("#showArticle_knowledgeMap") && $("#showArticle_knowledgeMap").length && $("#showArticle_knowledgeMap").length>0){jQuery.getJSON("/Jwxb/CN/lexeme/existsCnctstInArticle.do?articleID="+$("#articleId").val(), null, function(json){if(json.result){$("#showArticle_knowledgeMap").show();}});}});$(document).ready(function(){var metaDataStr = $.trim($("#article_metaJson").val());if(metaDataStr!=''){eval("window.metaData="+metaDataStr);}if(window.metaData && (!window.metaData.authorNotesCommon_cn && !window.metaData.authorNotesCorresp_cn && window.metaData.authorNotes_cn)){window.metaData.authorNotesCommon_cn = window.metaData.authorNotes_cn;}if(window.metaData && (!window.metaData.authorNotesCommon_en && !window.metaData.authorNotesCorresp_en && window.metaData.authorNotes_en)){window.metaData.authorNotesCommon_en = window.metaData.authorNotes_en;}var _nlmdtdXml = $("#article_nlmdtdXml").val();var magDir = _nlmdtdXml.replace(/\/[^\/]+$/,'');window.metaData.magDir = magDir;new mag_vue({ el: '#metaVue', //dataUrl: mag_currentQikanUrl() + '/EN/article/getRichHtmlJson.do?articleId='+$("#articleId").val(), data:window.metaData, loading:true, doTextFun:function(text){ return doDataJsonText(text); 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来源：2023年第9期
《菌物学报》期刊编辑部
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