作者：张宇薇,廖望仪,宋荣孝,彭龙,袁志林
单位：1 中国林业科学研究院 林木遗传育种国家重点实验室，北京 100091,2 南京林业大学林学院，江苏 南京 210037,3 中国林业科学研究院亚热带林业研究所，浙江 杭州 311400,4 浙江物产长乐创龄生物科技有限公司，浙江 杭州 311400
关键词：斜盖伞真菌,真菌诱导子,灵芝三萜,生物合成,诱导机制
基金：国家自然科学基金青年基金(31901290),中央级公益性科研院所基本科研业务费专项资金(CAFYBB2020QB002)
近年来，灵芝产业高速发展，但其主要药效成分灵芝三萜的含量较低，是影响其品质的重要原因，因此探索一种提高灵芝三萜含量的生物技术手段极具应用前景。本研究以6株斜盖伞Clitopilus spp.真菌为材料分别制备多种类型的真菌诱导子，将其添加到灵芝菌丝体液体摇瓶中，研究对灵芝菌丝体生物量和灵芝三萜积累的影响。其中，Clitopilus sp. HSL-YX-7-A的高压灭菌发酵液(HSL FB)、C. hobsonii NL-19的菌丝干粉(NL-19 DMP)、C. prunulus 84496的发酵液提取物(84496 FBE)和Clitopilus sp. HSL-YX-7-A的菌丝提取物(HSL ME)显著提高了灵芝菌丝体生物量和灵芝三萜含量，相对于对照组，生物量分别提高了450.09%、64.64%、46.97%和66.14%，灵芝三萜分别提高了53.01%、25.58%、25.17%和20.47%，灵芝三萜总产量则分别提高了585.15%、104.65%、86.43%和110.45%。进一步研究表明，经HSL FB、NL-19 DMP和HSL ME处理后，灵芝三萜生物合成途径关键酶基因hmgs、hmgr、mvd、fps、sqs和osc的表达量显著上调；但84496 FBE处理之后仅上调了hmgs和sqs的表达量。且6个基因的平均表达量与灵芝三萜总产量的提升率呈显著的正相关性(R2=0.972, P<0.05)。本研究证实了斜盖伞诱导子能够有效提升灵芝菌丝体生物量和灵芝三萜含量，其中C. hobsonii NL-19和Clitopilus sp. 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来源：2023年第6期
《菌物学报》期刊编辑部
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