作者：徐鸿雁,谢占玲,孟清,马德娟,孙灵芝
单位：1 青海大学生态环境工程学院,青海 西宁 810016,2 青海大学农林科学院,青海 西宁 810016
关键词：小海绵羊肚菌,镉,浓度拐点,镉吸收,抗氧化酶
基金：青海省科技计划国际合作专项(2021-HZ-802)
羊肚菌Morchella是全球广泛分布的食药用真菌,重金属镉(Cd)在羊肚菌中的积累受到越来越多的关注。然而,羊肚菌镉积累的机理尚不清楚。本研究通过在0-5.0 mg/L Cd浓度环境中培养小海绵羊肚菌Morchella spongiola,测定Cd胁迫下其菌丝生长速率、丙二醛(MDA)、过氧化氢(H2O2)、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、谷胱甘肽(GSH)、抗坏血酸(ASA)及细胞Cd积累量等生理生化指标,旨在明晰小海绵羊肚菌响应Cd毒害的抗氧化防御响应机理。结果表明随着Cd浓度的增加,小海绵羊肚菌菌丝生长呈现出“升-降-升-降”的双峰响应变化,其中0.15、0.90和1.50 mg/L为菌丝生长Cd浓度关键拐点。Cd胁迫导致的氧化损伤与其初始浓度呈现正相关,胁迫3 d时MDA和H2O2含量显示出较大提升,5.0 mg/L处理组MDA和H2O2含量比对照组分别高出5.80倍和6.08倍。胁迫浓度、胁迫时间对抗氧化系统的影响各异,SOD、POD活性随浓度增大而逐渐升高,在1.5-5.0 mg/L浓度范围内,SOD和POD酶活性分别增加了1.96倍和2.15倍;CAT和GSH-Px酶活在0.15 mg/L时达到最大值,之后增加Cd浓度酶活性被抑制;GSH和ASA含量在Cd胁迫初期呈浓度依赖性增加,胁迫5 d后表现二者含量先升高后降低。此外,小海绵羊肚菌胞外Cd积累量随着外源Cd浓度的提高而增加,而胞内Cd含量在浓度为1.5-5.0 mg/L范围内无明显增加。本研究首次系统研究了小海绵羊肚菌响应Cd胁迫浓度、胁迫时间的动态趋势与内在变化规律,不仅为羊肚菌与重金属相互作用关系研究奠定基础,也为羊肚菌用于Cd污染废水生物修复提供了理论依据。"/>template{display:none;}.mag-rich-xref-fn { cursor: pointer;}//长视频$(document).ready(function(){if($("#showLongArticleVideo") && $("#showLongArticleVideo").length && $("#showLongArticleVideo").length>0){mag_ajax_update({ele_id:'showLongArticleVideo',url:mag_currentQikanUrl() + "/CN/article/showLongArticleVideo.do?id="+$("#articleId").val()});}});//可视化图谱$(document).ready(function(){if($("#showArticle_knowledgeMap") && $("#showArticle_knowledgeMap").length && $("#showArticle_knowledgeMap").length>0){jQuery.getJSON("/Jwxb/CN/lexeme/existsCnctstInArticle.do?articleID="+$("#articleId").val(), null, function(json){if(json.result){$("#showArticle_knowledgeMap").show();}});}});$(document).ready(function(){var metaDataStr = $.trim($("#article_metaJson").val());if(metaDataStr!=''){eval("window.metaData="+metaDataStr);}if(window.metaData && (!window.metaData.authorNotesCommon_cn && !window.metaData.authorNotesCorresp_cn && window.metaData.authorNotes_cn)){window.metaData.authorNotesCommon_cn = window.metaData.authorNotes_cn;}if(window.metaData && (!window.metaData.authorNotesCommon_en && !window.metaData.authorNotesCorresp_en && window.metaData.authorNotes_en)){window.metaData.authorNotesCommon_en = window.metaData.authorNotes_en;}var _nlmdtdXml = $("#article_nlmdtdXml").val();var magDir = _nlmdtdXml.replace(/\/[^\/]+$/,'');window.metaData.magDir = magDir;new mag_vue({ el: '#metaVue', //dataUrl: mag_currentQikanUrl() + '/EN/article/getRichHtmlJson.do?articleId='+$("#articleId").val(), data:window.metaData, loading:true, doTextFun:function(text){ return doDataJsonText(text); 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来源：2022年第4期
《菌物学报》期刊编辑部
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