作者：郝转,张成花,黄秋菊,黄浩,李泰辉,王刚正,邓旺秋
单位：1 渭南师范学院环境与生命科学学院 陕西省河流湿地生态与环境重点实验室，陕西 渭南 714099,2 广东省科学院微生物研究所 华南应用微生物国家重点实验室 广东省菌种保藏与应用重点实验室，广东 广州 510075
关键词：广东虫草,CgDnaJ05,菌丝生长,原基形成
基金：国家自然科学基金(31900015),广东省科学院专项基金(2020GDASYL-20200103014),广州市科技计划(202102021051),广东省自然科学基金(2022A1515011083),陕西省教育厅科学研究计划(22JP022)
DnaJ蛋白(热激蛋白40)在机体生长发育和适应逆境环境过程中起着重要作用。以广东虫草GDGM30035为材料，克隆获得CgDnaJ05基因，CDS为2 361 bp，编码786个氨基酸，生物信息学分析结果表明CgDnaJ05蛋白含有DnaJ蛋白结构域，属于碱性、不稳定和亲水性蛋白质。以CgDnaJ05蛋白DnaJ保守结构域240 bp的反向互补片段为干扰片段，构建CgDnaJ05基因的双向启动子沉默载体；通过根癌农杆菌Agrobacterium tumefaciens介导转化法侵染广东虫草菌丝，筛选获得9个相对稳定的CgDnaJ05沉默转化子。qRT-PCR分析表明，转化子CgDnaJ05相对表达量下调至野生型菌株的20%-82%；与野生菌丝相比，转化子菌丝生长速度显著下降，且CgDnaJ05下调越明显，菌丝减缓速度越显著，同时，转化子原基的形成受到显著抑制。研究表明：广东虫草CgDnaJ05基因明显影响广东虫草菌丝生长和原基形成，对广东虫草生长发育具有重要的调控作用。"/>template{display:none;}.mag-rich-xref-fn { cursor: pointer;}//长视频$(document).ready(function(){if($("#showLongArticleVideo") && $("#showLongArticleVideo").length && $("#showLongArticleVideo").length>0){mag_ajax_update({ele_id:'showLongArticleVideo',url:mag_currentQikanUrl() + "/CN/article/showLongArticleVideo.do?id="+$("#articleId").val()});}});//可视化图谱$(document).ready(function(){if($("#showArticle_knowledgeMap") && $("#showArticle_knowledgeMap").length && $("#showArticle_knowledgeMap").length>0){jQuery.getJSON("/Jwxb/CN/lexeme/existsCnctstInArticle.do?articleID="+$("#articleId").val(), null, function(json){if(json.result){$("#showArticle_knowledgeMap").show();}});}});$(document).ready(function(){var metaDataStr = $.trim($("#article_metaJson").val());if(metaDataStr!=''){eval("window.metaData="+metaDataStr);}if(window.metaData && (!window.metaData.authorNotesCommon_cn && !window.metaData.authorNotesCorresp_cn && window.metaData.authorNotes_cn)){window.metaData.authorNotesCommon_cn = window.metaData.authorNotes_cn;}if(window.metaData && (!window.metaData.authorNotesCommon_en && !window.metaData.authorNotesCorresp_en && window.metaData.authorNotes_en)){window.metaData.authorNotesCommon_en = window.metaData.authorNotes_en;}var _nlmdtdXml = $("#article_nlmdtdXml").val();var magDir = _nlmdtdXml.replace(/\/[^\/]+$/,'');window.metaData.magDir = magDir;new mag_vue({ el: '#metaVue', //dataUrl: mag_currentQikanUrl() + '/EN/article/getRichHtmlJson.do?articleId='+$("#articleId").val(), data:window.metaData, loading:true, doTextFun:function(text){ return doDataJsonText(text); 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来源：2022年第11期
《菌物学报》期刊编辑部
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