作者：宋吉玲,周祖法,闫静,陆娜,程俊文,袁卫东,王伟科
单位：1.杭州市农业科学研究院 浙江 杭州 310024,2.浙江省林业科学研究院 浙江 杭州 310023
关键词：杨树桑黄,麸皮,超高效液相色谱-串联质谱（UPLC-MS/MS）技术,代谢组学
基金：浙江省科技厅项目(2016C02057),浙江省“三农六方”科技协作项目(CTZB-F190625LWZ-SNY1),杭州市农业与社会发展科研项目(20180416A02),杭州市财政项目(2020HNCT-11)
桑黄孔菌属Sanghuangporus是一类具有重要药用价值的大型真菌,目前被国际公认为抗肿瘤效果最好的药用真菌之一。本研究以添加麸皮栽培的杨树桑黄Sanghuangporus vaninii子实体为研究对象,基于液质联用技术的广泛靶向代谢组学研究,从杨树桑黄子实体中检测出355种代谢产物,差异代谢物86种,上调51种,下调35种,主要集中在氨基酸及其衍生物、核苷酸及其衍生物、糖及醇类、脂质、黄酮、生物碱、有机酸和酚酸等。KEGG代谢通路分析结果表明,差异代谢物富集于56条物质代谢通路,差异显著的有7条,分别为泛醌和其他萜类醌生物合成、次级代谢物的生物合成、色氨酸代谢、丙酸代谢、异喹啉生物碱生物合成和酪氨酸代谢。基于UPLC-MS/MS技术对杨树桑黄子实体进行代谢组学测定,确定麸皮的添加能够促进杨树桑黄次级代谢产物的合成和累积,为杨树桑黄的综合评价利用提供参考。"/>template{display:none;}.mag-rich-xref-fn { cursor: pointer;}//长视频$(document).ready(function(){if($("#showLongArticleVideo") && $("#showLongArticleVideo").length && $("#showLongArticleVideo").length>0){mag_ajax_update({ele_id:'showLongArticleVideo',url:mag_currentQikanUrl() + "/CN/article/showLongArticleVideo.do?id="+$("#articleId").val()});}});//可视化图谱$(document).ready(function(){if($("#showArticle_knowledgeMap") && $("#showArticle_knowledgeMap").length && $("#showArticle_knowledgeMap").length>0){jQuery.getJSON("/Jwxb/CN/lexeme/existsCnctstInArticle.do?articleID="+$("#articleId").val(), null, function(json){if(json.result){$("#showArticle_knowledgeMap").show();}});}});$(document).ready(function(){var metaDataStr = $.trim($("#article_metaJson").val());if(metaDataStr!=''){eval("window.metaData="+metaDataStr);}if(window.metaData && (!window.metaData.authorNotesCommon_cn && !window.metaData.authorNotesCorresp_cn && window.metaData.authorNotes_cn)){window.metaData.authorNotesCommon_cn = window.metaData.authorNotes_cn;}if(window.metaData && (!window.metaData.authorNotesCommon_en && !window.metaData.authorNotesCorresp_en && window.metaData.authorNotes_en)){window.metaData.authorNotesCommon_en = window.metaData.authorNotes_en;}var _nlmdtdXml = $("#article_nlmdtdXml").val();var magDir = _nlmdtdXml.replace(/\/[^\/]+$/,'');window.metaData.magDir = magDir;new mag_vue({ el: '#metaVue', //dataUrl: mag_currentQikanUrl() + '/EN/article/getRichHtmlJson.do?articleId='+$("#articleId").val(), data:window.metaData, loading:true, doTextFun:function(text){ return doDataJsonText(text); 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来源：2021年第3期
《菌物学报》期刊编辑部
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